Non cytotoxic, but antimicrobial to bacteria Non-resorbable Minimal leakage around the margins.
Eugenol Eugenol is found in the essential oil of cloves and has distinct properties that make it an important product to both food and drug industries.
In this experiment, eugenol was isolated from a sample Isolation of eugenol cloves using a series of techniques including steam distillation, solvent extraction, decantation, and evaporation. A simple-steam distillation apparatus was assembled and a mixture of water and cloves distilled to separate the eugenol from other clove components.
The Methylene Chloride was then decanted and evaporated yielding the isolated eugenol product. A percentage weight recovery of 3. The results suggested possible experimental error, and suggestions for future experiments are considered.
Introduction Eugenol is the primary chemical component comprising the oil of cloves, and has a wide range of uses, ranging from food flavoring to dental pharmaceuticals Bhimrao, et. Eugenol can be isolated from the clove oil using a series of techniques including steam distillation, the use of a solvent such as methylene chloride, and finally the decantaion and evaporation of that solvent which yields the isolated eugenol.
Steam distillation is a technique used to isolate the eugenol from cloves and avoid decomposition of the product. This is achieved by mixing two immiscible liquids and utilizing a higher vapor pressure, the sum of the two components, and thus a lower boiling point then either of the two components on their own.
The steam distillation will produce a mixture of eugenol and water which must be separated using a solvent such as methylene chloride. The solvent must subsequently be removed using a drying agent such as anhydrous sodium sulfate which will separate the mixture, allowing for the methylene chloride to be decanted and evaporated, which affords the liquid eugenol.
The product will then be weighed and weight percentage recovery will be determined. Fiber glass was used to insulate the distilling head during this procedure. The distilling flask was filled with The mixture was then distilled, at a rate of about one drop every two to three seconds.
Steam Distillation Apparatus Fig.
Methylene chloride works as a solvent for eugenol, as eugenol is highly soluble in it, and it separates the aqueous layer from the eugenol because it is denser than water 1.
The methylene chloride and distillate solution was then shaken vigorously and vented frequently to allow for separation. Once fully separated, the lower organic layer was then transferred to an Erlenmeyer flask using a Pasteur pipet.
This procedure was repeated using a fresh 5. Once all of the Methylene chloride was collected, it was then dried for 15 minutes using 1. This was conducted to remove any traces of water from the solution. One third of the dried solution excluding the drying agent was then decanted into a previously-weighed medium sized test tube.
The methylene chloride was then evaporated using air, until reduced to a small volume, in which another third was again decanted into the test tube and evaporated as previously stated.
This procedure was repeated until all of the Methylene chloride was transferred and evaporated, yielding the isolated eugenol product.
The test tube was then reweighed and percent yield recovery was calculated by taking the mass of isolated eugenol and dividing it by the mass of the sample of cloves. Results Following the steam distillation and solvent extraction of 3.
The empty test tube weighed in at This corresponds to a weight percentage recovery of 3. Discussion 1 Theory of Terpenes- The terpenes are hydrocarbons found in the essential oil of plants. They are arranged in repeating patterns of a five carbon unit which corresponds to a simple five-carbon compound called isoprene, leading to a diagnostic rule for terpenes called the isoprene rule, which states all terpenes must be divisible by isoprene units.
Eugenol is an example of a monoterpene containing 10 carbons which was successfully extracted from cloves in this experiment. Pavia, Terpenes hold potential interest especially in the fragrance and flavor industries, as well as in the pharmaceutical and chemical industries.IntroductionThroughout the ages, humans have relied on Nature for their basic needs for the production of food-stuffs, shelters, clothing, means of transportation, fertilizers, flavours and fragrances, and, not the least, medicines.
Isolation and Characterization of Eugenol from Cloves Alyssa Lynn P. Emata De La Salle University – Dasmarinas Dasmarinas City, Cavite, Philippines ABSTRACT The isolation of essential oil (eugenol) from botanical sources such as cloves undergoes a classic method of steam distillation.5/5(3).
Eugenol extraction from cloves using steam distillation The goal of this experiment is to isolate the natural product, eugenol, from cloves using the following procedure.
Chemistry Experiment #13 Isolation of Eugenol from Cloves g of eugenol was recovered from grams of cloves. This corresponds to a percent recovery of %: % Recovery = g of eugenol/ cloves used x = % Analysis The calculated percent yield of % is relatively low%(6).
In this experiment, eugenol was isolated from a sample of cloves using a series of techniques including steam distillation, solvent extraction, decantation, and evaporation. A simple-steam distillation apparatus was assembled and a mixture of water and cloves distilled to separate the eugenol .
The main volatile oil extracted from clove buds is eugenol. It is used in a variety of ways in traditional medicine and can be isolated successfully using a variety of methods.
Isolation was performed using steam distillation. A 3. 0% yield of eugenol oil was observed during the experiment. Introduction Eugenol is an essential oil [ ].